GlowCas9 Protein:
Scientists from Kolkata based Bose institute has created GlowCas9 which can track gene editing.
- It is a CRISPR protein that lights up while performing gene editing.
- It is a bioluminescent version of Cas9 that glows inside cells.
- GlowCas9 is created by fusing Cas9 with a split nano-luciferase enzyme derived from deep-sea shrimp proteins.
- Properties of GlowCas9 Protein:
- The GlowCas9 is very stable and maintains its structure and activity at higher temperatures compared to the conventional enzyme.
- It glows inside cells, allowing for real-time monitoring of CRISPR operations
- Working of GlowCas9 Protein:
- The split nano-luciferase enzyme pieces reconnect when Cas9 folds correctly, producing light.
- This glowing activity allows scientists to monitor CRISPR operations in living cells, tissues, and even plant leaves—without harming them.
- Benefits of GlowCas9 Protein
- It can also increase the precision of Homology-Directed Repair (HDR).
- Homology-Directed Repair (HDR) is a DNA repair process crucial for fixing hereditary mutations that are linked to genetic diseases like sickle cell anaemia, muscular dystrophy and so on.
- GlowCas9 pioneers the emerging field of visualizing molecular gene therapy in motion.
