Diffraction Limit:
The resolution limit of an optical instrument that uses light is constrained by the diffraction limit, a fundamental boundary that prevents improvement beyond a certain point.This diffraction limit affects the instrument’s ability to distinguish between two close objects. Due to the diffraction limit, scientists could use the light microscope to see cells but not the proteins inside them or a virus attacking them. However, optical microscopes can see inside cells and even things as small as atoms. This technique is called super-resolution microscopy, and it is not bound by the diffraction limit.
