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What Is Ct value In RT-PCR tests

Ct value’ In RT-PCR tests:

Among various scientific terms that the Covid-19 pandemic has made part of the public vocabulary, one is the ‘Ct value’ in RT-PCR tests for determining whether a patient is positive for Covid-19.

  • Short for cycle threshold, Ct is a value that emerges during RT-PCR tests, the gold standard for detection of the SARS-CoV-2 coronavirus.
  • According to the ICMR advisory, the Ct value of an RT-PCR reaction is the number of cycles at which fluorescence of the PCR product is detectable over and above the background signal.
  • The Ct value refers to the number of cycles after which the virus can be detected. If a higher number of cycles is required, it implies that the virus went undetected when the number of cycles was lower.
  • The lower the Ct value, the higher the viral load — because the virus has been spotted after fewer cycles.
  • According to the ICMR, a patient is considered Covid-positive if the Ct value is below 35. In other words, if the virus is detectable after 35 cycles or earlier, then the patient is considered positive.
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